Protective effect of methanolic extract of Areca catechu nut on ethanol withdrawal symptoms in mice

Abstract Background The purpose of the current study was to examine potential impact a methanolic extract Areca catechu nut (MAN) on handling-induced convulsions (HIC), anxiety and anhedonia behaviour alcohol-withdrawn mice. 30 female Swiss albino mice were divided into 5 groups, each with 6 animals. Group 1 (saline withdrawal) received saline during 3-day alcohol/saline induction phase, while other 4 groups (alcohol 20% v/v ethanol (1.25 ml/100 g body weight, i.p.; made from absolute 79.9 ml + 0.1 fomepizole, an alcohol dehydrogenase inhibitor). Day four (test day) involved studying convulsions; open field test (OFT), elevated plus maze (EPM), marble burying (MBT) for anxiety; 24-h sucrose preference (SPT) in On day, I II withdrawal oral treatments 1% w/v sodium carboxyl methylcellulose h prior behavioural testing. III injection diazepam (1 mg/kg, i.p., min prior) IV V treated two different doses MAN (50 100 p.o.) test. Results At 50 p.o., significantly reduced handling anxiety, had anti-anhedonic effect using various evaluation criteria, such as convulsion score no. central peripheral line crossings % entries time spent arms marbles buried (MBT), intake ratio Conclusion In undergoing withdrawal, greatly lessens convulsions, depression symptoms. Graphical